I'm unable to use htaccess. Do I need special settings?

To be able to use a .htaccess file, the configuration of apache for that specific site needs to specify "AllowOverride All".
It's a directory keyword so you usually do it like this:

I have it, but it doesn't work

And you actually named the file .htaccess (the dot in front has to be there) and you are sure it has no errors?
Those files aren't scanned when you start your server, so if they have an error, I think they are just ignored.

Yes, I use the same file with browser and it works perfect

Did you set run mode to "SRV" or "3rd party web server"?
.htaccess is a feature of Apache and only Apache. In order to work with Apache you have to set run mode to 3rd party web server and set the project properties appropriately.

Thanks, now it works perfect. Problem was, that I didn't have debugger in Apache

For all other with same problem like me, I recommend to visit this site and download the wizard:
http://www.nusphere.com/products/dbg_wizard_download.htm